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It is used for the diagnostic test, to determine the ability of the organism to split tryptophan into indole and alpha-aminopropionic acid by hydrolytic activity of bacteria that express tryptophanase enzyme (3).
Discrete bacterial colonies on solid medium. Tube method. 1% tryptophan or peptone broth. Kovác's reagent (for use with broth cultures).
Remel Indole (Kovacs') is a reagent recommended for use in qualitative Previous tests for the detection of indole production required a xylene.
KOVACS INDOLE REAGENT
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