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The main use of Bouin's fluid is the fixation of lymph nodes, prostate and kidneys biopsies. It is a very good fixative when soft and delicate tissue structures must be preserved on the other hand it is not advisable to use it to fix the tissues for electron microscopy.
Bouin solution, or Bouin's solution, is a compound fixative used in histology. It was invented by French biologist Pol Bouin and is composed of picric acid, acetic acid and formaldehyde in an aqueous solution.
Bouin's fixative consists of picric acid, formaldehyde and acetic acid. The primary use of Bouin's fixative is for lymph nodes, prostate biopsies and kidney biopsies however, it can also be used for decalcifying bones with the addition of formic acid.
Bouin's Solution is a yellow liquid made up of formaldehyde, picric acid, and acetic acid. It is classified as a category 1A carcinogen due to the presence of Formaldehyde. If the solution dries out, the picric acid can be explosive.
BOUIN’S FLUID
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